Anti-EN1 / Engrailed 1 抗体 [EPR3282] (ab108598)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3282] to EN1 / Engrailed 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EN1 / Engrailed 1 antibody [EPR3282]
EN1 / Engrailed 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3282] to EN1 / Engrailed 1 -
由来種
Rabbit -
特異性
We have data to suggest no cross-reactivity with EN2/Engrailed 2, please contact us if you require further details/experimental data
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アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- U87-MG, SH-SY5Y, 293T and Human fetal brain lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3282 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108598の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). |
ターゲット情報
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配列類似性
Belongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2019 Human
- Entrez Gene: 13798 Mouse
- Entrez Gene: 685360 Rat
- Omim: 131290 Human
- SwissProt: Q05925 Human
- SwissProt: P09065 Mouse
- Unigene: 271977 Human
- Unigene: 2657 Mouse
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別名
- En 1 antibody
- EN1 antibody
- Engrailed 1 antibody
see all
画像
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All lanes : Anti-EN1 / Engrailed 1 antibody [EPR3282] (ab108598) at 1/1000 dilution
Lane 1 : U87-MG lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
All lanes : Anti-EN1 / Engrailed 1 antibody [EPR3282] (ab108598) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108598 は 2 報の論文で使用されています。
- Shen J et al. MicroRNA-34c promotes neuronal recovery in rats with spinal cord injury through the C-X-C motif ligand 14/Janus kinase 2/signal transducer and activator of transcription-3 axis. Chin Med J (Engl) 133:2177-2185 (2020). PubMed: 32826607
- Wu SY et al. Opposing Functions of BRD4 Isoforms in Breast Cancer. Mol Cell 78:1114-1132.e10 (2020). PubMed: 32446320