Application
Western blot
Sample
Mouse Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
BRAIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (horse serum)
Dilution
1/100
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
LAS
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 28 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Kyungjoo Seong
Verified customer
投稿 Nov 30 2016