Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (differentiated HL60)
Total protein in input
1.5e+007 cells
Specification
differentiated HL60
Immuno-precipitation step
Protein A/G
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s)
Western Blot antibody
Name
Non-Abcam antibody was used: Alexa Fluor 680 donkey anti–goat IgG antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Additional data
Additional Notes
Immunoprecipiated with polyclonal goat anti-ELMO1 antibody 1.5 ug/sample (about 400 ug total protein). Immunoblotted with polyclonal rabbit anti-Dock2 (Lab-made) or anti-ELMO1 antibody.
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投稿 Apr 05 2007