Anti-EIF3F 抗体 (ab64177)
Key features and details
- Rabbit polyclonal to EIF3F
- Suitable for: IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EIF3F antibody
EIF3F 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to EIF3F -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Cynomolgus monkey -
免疫原
Synthetic peptide:
ARVIGTLLGTVD
, corresponding to amino acids 114-125 of Human EIF3F -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64177の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 2.5 µg/ml.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use a concentration of 2.5 µg/ml. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. -
配列類似性
Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain. -
ドメイン
The MPN domain mediates deubiquitinating activity. -
翻訳後修飾
Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8665 Human
- Entrez Gene: 293427 Rat
- Omim: 603914 Human
- SwissProt: Q4R5B8 Cynomolgus monkey
- SwissProt: O00303 Human
- Unigene: 516023 Human
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別名
- Deubiquitinating enzyme eIF3f antibody
- eIF 3 epsilon antibody
- eIF-3-epsilon antibody
see all
画像
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ab64177 at 2.5µg/ml staining EIF3F in human brain cerebellum by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
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ICC/IF image of ab64177 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64177, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab64177 は 3 報の論文で使用されています。
- Theodoridis PR et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33568529
- Parajuli P et al. Twist1 Activation in Muscle Progenitor Cells Causes Muscle Loss Akin to Cancer Cachexia. Dev Cell 45:712-725.e6 (2018). PubMed: 29920276
- Lang CH et al. BCATm deficiency ameliorates endotoxin-induced decrease in muscle protein synthesis and improves survival in septic mice. Am J Physiol Regul Integr Comp Physiol 299:R935-44 (2010). PubMed: 20554928