Application
Western blot
Sample
Rabbit Cell lysate - whole cell (RK13 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
200000 cells
Specification
RK13 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in TBS-Tween-5%BSA
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce West DUra
Exposure
6 second(s)
Bands
Specific: 37 kDa
Positive control
Cells treated for 30min with 50´M sodium arsenite
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 25 2017