Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (S2R+ cells)
Loading amount
25 µg
Specification
S2R+ cells
Treatment
0.5mM arsenite for 1hr
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% normal horse serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: donkey-anti-rabbit-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 37 kDa
Additional data
PubMed ID
19661161: View
Additional Notes
I was examining phosphorylation of eIF2alpha under various conditions, using phospho-specific eIF2alpha antibody Ab32157 (see Abreview for Ab32157). I used this antibody as a loading control for total eIF2alpha levels in knockdown and arsenite-treated cells. Total eIF2alpha is strongly detected in all lanes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Natalie Farny
Verified customer
投稿 Sep 28 2009