Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW 264.7 murine macrophage)
Loading amount
10 µg
Specification
RAW 264.7 murine macrophage
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti mouse HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Additional data
Additional Notes
Blocked with Pierce Starting Block- PBS with 0.1% Tween-20.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 23 2006