Anti-EIF2S1 抗体 [EIF2S1A2B8] (ab50733)
Key features and details
- Mouse monoclonal [EIF2S1A2B8] to EIF2S1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-EIF2S1 antibody [EIF2S1A2B8]
EIF2S1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EIF2S1A2B8] to EIF2S1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant Human EIF2S1
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ポジティブ・コントロール
- HEK293 and NIH3T3 whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab50733 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
EIF2S1A2B8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50733の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/1.
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特記事項 |
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WB
1/50. Predicted molecular weight: 36 kDa. |
ICC/IF
1/1. |
ターゲット情報
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機能
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
配列類似性
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
翻訳後修飾
Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
細胞内局在
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Omim: 603907 Human
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
- Unigene: 151777 Human
- Unigene: 196220 Mouse
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別名
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
画像
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Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
ab50733 at 1/1 dilution staining human EIF2S1 in HeLa Cells by Immunocytochemistry. A 1/200 dilution of Alexa Fluor 488 anti-mouse IgG is used as a secondary.
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Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab50733 は 1 報の論文で使用されています。
- Yu Y et al. PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection. PLoS Pathog 9:e1003266 (2013). WB ; Human . PubMed: 23592989