Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (sperm)
Total protein in input
1e+008 cells
Specification
sperm
Immuno-precipitation step
Protein A/G
Other product details
Dilution
1/50
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: homogenization buffer
Additional data
Additional Notes
Using this antibody for IP it gave very good results in mouse sperm. the band that was seen was ~170kDa. When a whole cell lysate was tested (WB) the band was not seen very clearly on the blot. dilution of 1:1000 was used for the WB over night (12-15h) in 4 degrees celcius .
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Yael Jaldety
Verified customer
投稿 Jun 24 2011