Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (4-10%)
Sample
Orthopoxvirus Serum (BHK-21 cells)
Specification
BHK-21 cells
Treatment
Infected with Western Reserve Strain of vaccinia virus
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Concentration
1 µg/ml
Incubation time
2 hour(s) and 0 minute(s) · Diluent: Room Temp
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase conjugated Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2500
Detection
Detection method
ECL
Bands
Specific: 28 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Uninfected BHK-21 cells
Additional data
Additional Notes
Lane 1: Supernatant from BHK-21 cells infected with vaccinia virus WR
Lane 2: Supernatant from uninfected BHK-21 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. B Ward
Verified customer
投稿 Oct 22 2013