Application
Western blot
Sample
Human Cell lysate - whole cell (293T, U2OS and MCF7 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
293T, U2OS and MCF7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk 0.1% Tween-20 in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal West Dura
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 95 kDa
Additional data
Additional Notes
Excellent antibody - clean and specific.
Lane order: 1, 293T 2, U2OS 3, MCF-7
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 12 2007