Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (hippocampal neuronal culture)
Specification
hippocampal neuronal culture
Permeabilization
Yes - 0.2% Triton
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% NGS, 0.2% Triton
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse IgG Alexa 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
Additional data
Additional Notes
I have performed ICC staining side by side for neuronal culture using a rabbit EEA1 Antibody and it worked well. So the problem should be in the permeablization method.
Abcam response
We would like to thank the user for providing the additional data and for using the AbTrial program. As we have not tested this antibody in mouse samples, we would welcome any additional information that other researchers might have. This customer has received a discount code valued at the original purchase price of this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 12 2013