Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP-1)
Specification
THP-1
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS+ tween 20 +BSA 1%
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594 + Gold 1.4nm
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594 + Gold 1.4nm
Dilution
1/500
Additional data
Additional Notes
Red staining for EEA1 (Early endosome marker)
Green staining for StarD5 (mainly cytosolic staining)
Green staining for StarD5 (mainly cytosolic staining)
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投稿 Apr 01 2010