Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Cortical cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - 0.1% Triton X
Specification
Cortical cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 35°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
This mouse brain tissue was PFA perfusion fixed, cut 20 microns thick, and pre-mounted on slides. I did not post fix my tissue. I used sodium citrate heat retrieval method. I blocked with both BSA (5%) and Serum. I got very good somatic staining with this two step process. This image shows LPAR1/ EDG2 (red) and Dapi (blue) at 40x magnification in mouse cortex.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 10 2013