Application
Western blot
Sample
Cow Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/750
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Anti - Rabbit IgG,
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 62kDa kDa Non-specific: 100kDa kDa
Additional data
Additional Notes
lane 1: 15ug cow muscle, Lane 2: 30ug cow muscle, and Lane 3 : 60ug cow muscle
I just verified this antibody with different loading amount and next I will do blocking test
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 25 2017