Anti-ELF1 抗体 (ab64937)
Key features and details
- Rabbit polyclonal to ELF1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ELF1 antibody
ELF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ELF1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ELF1 (C terminal).
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ポジティブ・コントロール
- A549 cells and cell extracts from 293 and HuvEc cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64937の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500 - 1/1000.
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WB |
1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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特記事項 |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
ターゲット情報
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機能
Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. -
組織特異性
In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1997 Human
- Omim: 189973 Human
- SwissProt: P32519 Human
- Unigene: 135646 Human
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別名
- E74 like factor 1 antibody
- E74-like factor 1 antibody
- ELF 1 antibody
see all
画像
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This image shows A549 cells stained with ab64937 at 1/500 dilution; with and without the immunising peptide.
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All lanes : Anti-ELF1 antibody (ab64937) at 1/500 dilution
Lane 1 : extracts from 293 cells (5-30µg total protein)
Lane 2 : extracts from HuvEc cells (5-30µg total protein)
Lane 3 : extracts from HuvEc cells (5-30µg total protein) and 5-10µg of the immunising peptide.
Predicted band size: 67 kDa
Observed band size: 67 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab64937 は 3 報の論文で使用されています。
- Cheng M et al. Transcription Factor ELF1 Activates MEIS1 Transcription and Then Regulates the GFI1/FBW7 Axis to Promote the Development of Glioma. Mol Ther Nucleic Acids 23:418-430 (2021). PubMed: 33473327
- Qiao C et al. SNHG17/miR-384/ELF1 axis promotes cell growth by transcriptional regulation of CTNNB1 to activate Wnt/ß-catenin pathway in oral squamous cell carcinoma. Cancer Gene Ther N/A:N/A (2021). PubMed: 33531646
- Ben Khalaf N et al. The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling. Mol Immunol 111:209-219 (2019). PubMed: 31096062