製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to E2F6
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    NPQQSEELLEVSN

    corresponding to C terminal amino acids 268 - 281 of Human E2F6, with a Cysteine residue linker (NP_937987.2).

  • ポジティブ・コントロール
    • Human skeletal muscle lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab99456 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32 kDa).

ターゲット情報

  • 機能
    Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
  • 組織特異性
    Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
  • 配列類似性
    Belongs to the E2F/DP family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E2F 6 antibody
    • E2F binding site modulating activity protein antibody
    • E2F transcription factor 6 antibody
    • E2F transcription factor 6 isoform 1 antibody
    • E2F-6 antibody
    • E2F6 antibody
    • E2F6_HUMAN antibody
    • EMA antibody
    • MGC111545 antibody
    • Transcription factor E2F6 antibody
    see all

画像

  • Anti-E2F6 antibody (ab99456) at 1 µg/ml + Human skeletal muscle lysate, in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab99456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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