Anti-E2F6 抗体 (ab53061)
Key features and details
- Rabbit polyclonal to E2F6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-E2F6 antibody
E2F6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to E2F6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human E2F6 aa 141-190.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53061の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
組織特異性
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
配列類似性
Belongs to the E2F/DP family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1876 Human
- Entrez Gene: 50496 Mouse
- Omim: 602944 Human
- SwissProt: O75461 Human
- SwissProt: O54917 Mouse
- Unigene: 603093 Human
- Unigene: 23296 Mouse
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別名
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
画像
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All lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution
Lane 1 : Extracts of HepG2 cells
Lane 2 : Extracts of HepG2 cells treated with peptide
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Immunohistochemistry analysis of paraffin-embedded human brain tissue labeling E2F6 with ab53061. The picture on the right is blocked with the synthesized peptide.
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Anti-E2F6 antibody (ab53061) at 1/500 dilution + NIH/3T3 cell lysate
Predicted band size: 31 kDa -
Anti-E2F6 antibody (ab53061) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 31 kDa -
All lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )
Lane 1 : HeLa cells
Lane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cells
Secondary
All lanes : HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Blocking Step: 4% Milk for 1 hour at 22°C
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab53061 は 9 報の論文で使用されています。
- Dahlet T et al. E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. Nat Commun 12:3582 (2021). PubMed: 34117224
- Mathsyaraja H et al. Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness. Elife 10:N/A (2021). PubMed: 34236315
- Liu L et al. The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma. Front Oncol 11:648152 (2021). PubMed: 34381702
- Zhao H et al. LncRNA TMPO-AS1 promotes LCN2 transcriptional activity and exerts oncogenic functions in ovarian cancer. FASEB J 34:11382-11394 (2020). PubMed: 32692467
- Roelofs PA et al. Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B. Elife 9:N/A (2020). PubMed: 32985974
- Shi X et al. Nuclear receptor TLX regulates islet beta cell proliferation via E2F6. Biochem Biophys Res Commun 513:560-566 (2019). PubMed: 30981507
- Scelfo A et al. Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities. Mol Cell 74:1037-1052.e7 (2019). PubMed: 31029542
- Roy-Chaudhuri B et al. Regulation of microRNA-mediated gene silencing by microRNA precursors. Nat Struct Mol Biol 21:825-32 (2014). PubMed: 25086740
- Velasco G et al. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc Natl Acad Sci U S A 107:9281-6 (2010). WB, IP ; Human . PubMed: 20439742