Application
Western blot
Sample
Human Cell lysate - whole cell (HT29 tumour cell line)
Loading amount
50 µg
Specification
HT29 tumour cell line
Treatment
50um etoposide for 6 hr
Gel Running Conditions
Reduced Denaturing (10% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TNT buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 55, 60 kDa
Additional data
Additional Notes
Treatment with either etoposide or doxorubicin caused the induction of two bands. With no treatment there was only one slower migrating band visible
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Paul Eve
Verified customer
投稿 Apr 25 2008