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Product Name 

E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag

Product type 

Lysates

Description 

E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag

Tested applications 
(see key)

WB

Application notes 
(see key)

Recommended dilutions
WB: Use 0.5 - 2.0 µl for 10 - 12% gels.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Arabidopsis / Miscellaneous
Tags & Cell Markers >> Epitope Tags >> Other Tags >> HSV Tag
Tags & Cell Markers >> Epitope Tags >> Other Tags >> S Tag
Tags & Cell Markers >> Epitope Tags >> Other Tags >> VSV-G Tag
Tags & Cell Markers >> Epitope Tags >> Other Tags >> E tag
Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli
Tags & Cell Markers >> Epitope Tags >> T7 Tag
Tags & Cell Markers >> Epitope Tags >> V5 Tag
Tags & Cell Markers >> Epitope Tags >> Glu-Glu Tag
Tags & Cell Markers >> Epitope Tags >> DDDDK Tag
Tags & Cell Markers >> Epitope Tags >> Myc Tag
Tags & Cell Markers >> Epitope Tags >> HA Tag

Relevance 

Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

Excellent as a loading control for Western blotting.

 

E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395)の別名

Purity 

Whole Cell Lysate

Storage buffer 

Preservative: None
Constituents: 1 x SDS-PAGE sample loading buffer

Purification notes 

E. coli were lysed with SDS-PAGE sample loading dye, boiled at 95°C for 10 minutes, then centrifuged at 13,000 g for 15 minutes.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of:

T7
HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His

Tag sequences:
T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
GluGlu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH

 

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Search PubMed (MEDLINE) for references to E. coli Positive Control

 

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