Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Excellent as a loading control for Western blotting.
E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395)の別名
Purity
Whole Cell Lysate
Storage buffer
Preservative: None Constituents: 1 x SDS-PAGE sample loading buffer
Purification notes
E. coli were lysed with SDS-PAGE sample loading dye, boiled at 95°C for 10 minutes, then centrifuged at 13,000 g for 15 minutes.
Form
Liquid
Concentration
0.500 mg/ml
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Notes
This E.coli lysate contains a 41 kd recombinant protein expressing 11 commonly used Tags which are fused to the N-terminus of the protein in the order of:
T7 HSV C-Myc VSV-g Glu-Glu V5 E-tag FLAG (DDDDK) S-tag HA 6x His