Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Tammar wallaby Tissue sections (Testis- Day 1 pouch young and Day 10 PY)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris
Permeabilization
No
Specification
Testis- Day 1 pouch young and Day 10 PY
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x PBS + 0.1%BSA
Concentration
5 µg/ml
Secondary antibody
Dilution
1/800
Name
Non-Abcam antibody was used: Goat anti-mouse
Host species: Tammar wallaby
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Tammar wallaby
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your valuable feedback. ab76055 has not been previously tested in Tammar wallaby sample. It is possible that this antibody may not be suitable for use in this species. We welcome other Abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 03 2018