Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
I have tried this antibody at dilutions at 1:50 1:100, 1:250, 1:500, and 1:1000, but was unable to detect a clear signal above backround level. Phalloidin (blue) was used as a counterstain.
Abcam response
Thank you for sharing this data with us. In the abcam lab, ab40772 has only been tested on mouse by western blot. However, based on the data shown here (and in other abreviews), ab40772 is not suitable for IHC on mouse tissues. We would recommend using ab11512 for IHC-Fr on mouse and welcome feedback from other researchers using these antibodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
投稿 Jul 12 2016