Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7)
Specification
MCF-7
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
0.1% BSA + 1% goat serum in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 0.1% BSA + 1% goat serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor goat anti-rabbit IgG 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 28 2013