Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muscle Fiber)
Specification
Muscle Fiber
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 2°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 488 Goat-Anti Rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Good staining in the subsarcolemmic area. The Isotype Control was negative almost at background level.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 19 2012