Anti-Dynorphin A 抗体 (ab82509)
Key features and details
- Rabbit polyclonal to Dynorphin A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dynorphin A antibody -
製品の詳細
Rabbit polyclonal to Dynorphin A -
由来種
Rabbit -
特異性
Replenishment batches of ab82509 are tested in IHC-P. Previous batches were additionally validated in ICC/IF and WB. These applications are still expected to work and are covered by our Abpromise guarantee.
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アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Guinea pig, Cow, Pig, Monkey -
免疫原
Synthetic peptide corresponding to Human Dynorphin A aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab91634) -
ポジティブ・コントロール
- This antibody gave a positive signal in human brain membrane tissue lysate. IHC-P: FFPE mouse dorsal root ganglion.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
ターゲット情報
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関連性
Dynorphins are a class of peptides produced by many different populations of neurons, which has some opiate like activity. It is thus classed as an endogenous opioid peptide. The dynorphins, which include dynorphin A, dynorphin B, alpha and beta neoendorphin, and big dynorphin, are all the products of a single gene, 'preprodynorphin'. Dynorphin is produced in many different parts of the brain, including the hypothalamus, the hippocampus and the spinal cord, and has many different physiological actions, depending upon its site of production. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. As such, it may help some individuals against addiction. Blocking dynorphin may help alleviate depression. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 5173 Human
- Entrez Gene: 18610 Mouse
- Entrez Gene: 29190 Rat
- Omim: 131340 Human
- SwissProt: P01213 Human
- SwissProt: O35417 Mouse
- SwissProt: P06300 Rat
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別名
- ADCA antibody
- Beta neoendorphin dynorphin antibody
- Dyn A17 antibody
see all
画像
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IHC image of Dynorphin A staining in formalin fixed, paraffin embedded mouse dorsal root ganglion tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82509, 10µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Anti-Dynorphin A antibody (ab82509) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 15 minutes -
ICC/IF image of ab82509 stained PC-12 (Rat adrenal gland pheochromocytoma) cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
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IHC image of Dynorphin A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82509, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
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Anti-Dynorphin A antibody (ab82509) at 1/1000 dilution + Mouse synaptosome from hippocampal stratum lucidum tissue lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)
Exposure time: 15 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab82509 は 3 報の論文で使用されています。
- Cattani D et al. Perinatal exposure to a glyphosate-based herbicide causes dysregulation of dynorphins and an increase of neural precursor cells in the brain of adult male rats. Toxicology 461:152922 (2021). PubMed: 34474092
- McAlpine CS et al. Sleep modulates haematopoiesis and protects against atherosclerosis. Nature 566:383-387 (2019). PubMed: 30760925
- Podolnikova NP et al. The opioid peptide dynorphin A induces leukocyte responses via integrin Mac-1 (aMß2, CD11b/CD18). Mol Pain 11:33 (2015). PubMed: 26036990