Anti-p50 dynamitin 抗体 (ab124285)
Key features and details
- Rabbit polyclonal to p50 dynamitin
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-p50 dynamitin antibody
p50 dynamitin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p50 dynamitin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis, Elephant -
免疫原
Synthetic peptide corresponding to Human p50 dynamitin aa 1-50 (N terminal).
Database link: NP_006391.1 -
ポジティブ・コントロール
- Jurkat, 293T, HeLa and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab124285 was affinity purified using an epitope specific to p50 dynamitin immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000.
|
|
IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. -
配列類似性
Belongs to the dynactin subunit 2 family. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10540 Human
- Entrez Gene: 69654 Mouse
- Entrez Gene: 299850 Rat
- Omim: 607376 Human
- SwissProt: Q3ZCF0 Cow
- SwissProt: Q13561 Human
- SwissProt: Q99KJ8 Mouse
- SwissProt: Q6AYH5 Rat
see all -
別名
- 50 kD dynein associated polypeptide antibody
- 50 kDa dynein associated polypeptide antibody
- 50 kDa dynein-associated polypeptide antibody
see all
画像
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All lanes : Anti-p50 dynamitin antibody (ab124285) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds -
ab124285 at 1 µg/ml staining p50 dynamitin in Jurkat whole cell lysate immunoprecipitated using ab124285 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 44 kDa. Exposure time: 3 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab124285 は論文での使用が確認できていません。