Anti-Dux4 抗体 [E5-5] (ab124699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E5-5] to Dux4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Dux4 antibody [E5-5]
Dux4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E5-5] to Dux4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human testis tissue lysates, HT-1080, PC-3, Saos-2, HeLa, 293T and A549 whole cell lysates.
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特記事項
For details on the development of this clone, please refer to Geng et al. Hybridoma (Larchmt). 2011 Apr; 30(2): 125–130.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E5-5 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse testis normal tissue lysate - total protein (ab29289)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ターゲット情報
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関連性
DUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 100288687 Human
- Omim: 606009 Human
- SwissProt: Q9UBX2 Human
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別名
- Double homeobox 4 antibody
- Double homeobox protein 10 antibody
- Double homeobox protein 4 antibody
see all
画像
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All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : HFF-1 (Human skin fibroblast) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 60 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM /TBSTThe expression profile observed in HFF-1 is consistent with the literature (PMID: 30420784).
Negative control : HFF-1 (PMID: 30420784) -
All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)
Lane 1 : HT1080 (human fibrosarcoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : Saos-2 (human osteosarcoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)
Lane 1 : Human testis lysate
Lane 2 : HT1080 (human fibrosarcoma cell line) cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 45 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab124699 は 30 報の論文で使用されています。
- Gros M et al. Identification of Serum Interleukin 6 Levels as a Disease Severity Biomarker in Facioscapulohumeral Muscular Dystrophy. J Neuromuscul Dis 9:83-93 (2022). PubMed: 34459413
- Hangul C et al. Analysis of DUX4 Expression in Bone Marrow and Re-Discussion of DUX4 Function in the Health and Disease. Turk Patoloji Derg 38:219-226 (2022). PubMed: 34854471
- Ashoti A et al. Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen. PLoS One 17:e0263262 (2022). PubMed: 35176052
- Masteika IF et al. Downstream events initiated by expression of FSHD-associated DUX4: Studies of nucleocytoplasmic transport, γH2AX accumulation, and Bax/Bak-dependence. Biol Open 11:N/A (2022). PubMed: 35191484
- Vuoristo S et al. DUX4 is a multifunctional factor priming human embryonic genome activation. iScience 25:104137 (2022). PubMed: 35402882