Application
Immunohistochemistry (PFA fixed)
Sample
Mouse Cell (Brain)
Specification
Brain
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Milk TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Dilution
1/200
Additional data
Additional Notes
1. Immunofluorescence staining on mouse embryonic brain tissue, stage 10.5.
2. Other blocking agents used (serum and PBS-Triton) with similar results.
3. An epitope retrieval protocol was conducted in order to enhance the binding sites for the antibody however, results remained inconclusive.
4. The antibody was used on cells transfected with DSCAM, also with inconclusive results.
*Note: additional photos and data available on request
2. Other blocking agents used (serum and PBS-Triton) with similar results.
3. An epitope retrieval protocol was conducted in order to enhance the binding sites for the antibody however, results remained inconclusive.
4. The antibody was used on cells transfected with DSCAM, also with inconclusive results.
*Note: additional photos and data available on request
Abcam response
Thank you for your honest feedback. This antibody has not yet been tested in fixed tissue staining, so we are unsure if this antibody is suitable for these purposes. Perhaps the epitope does not survive fixation, however more testing would need to be conducted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Minkyung Kim
Verified customer
投稿 Jun 04 2010