Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Embryonic day 6 developing brain)
Specification
Embryonic day 6 developing brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/2500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300
Additional data
Additional Notes
Tissue was fixed, in 4% Formalin (from commercial liquid concentrate) in Dulbecco A PBS (from tablet). Duration of immersion-fixation was 5 days, before processing to Pwax via Industrial Methylated spirit and xylene. Section thickness was 5 microns. Section in picture was counterstained using Gills Haematoxylin II, before dehydration in alcohol, clearing in Xylene and mounting using DPX.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 05 2007