Anti-Dopamine beta Hydroxylase 抗体 (ab96615)
Key features and details
- Rabbit polyclonal to Dopamine beta Hydroxylase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Dopamine beta Hydroxylase antibody
Dopamine beta Hydroxylase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dopamine beta Hydroxylase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 8 and 290 of Dopamine beta Hydroxylase (NP_000778).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96615の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 69 kDa.
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IHC-P |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 69 kDa. |
IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Conversion of dopamine to noradrenaline. -
パスウェイ
Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1. -
関連疾患
Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. -
配列類似性
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1621 Human
- Omim: 609312 Human
- SwissProt: P09172 Human
- Unigene: 591890 Human
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別名
- dbh antibody
- DBM antibody
- Dopamine beta hydroxylase antibody
see all
画像
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mmunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded SW480 xenograft, using ab96615 at a 1/500 dilution.
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All lanes : Anti-Dopamine beta Hydroxylase antibody (ab96615) at 1/2000 dilution
Lane 1 : SH-SY5Y whole cell extract
Lane 2 : 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDaSeparated by 7.5% SDS-PAGE.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab96615 は 3 報の論文で使用されています。
- Gaceb A et al. An In Vitro Partial Lesion Model of Differentiated Human Mesencephalic Neurons: Effect of Pericyte Secretome on Phenotypic Markers. J Mol Neurosci 70:1914-1925 (2020). PubMed: 32472394
- Saxton SN et al. Role of Sympathetic Nerves and Adipocyte Catecholamine Uptake in the Vasorelaxant Function of Perivascular Adipose Tissue. Arterioscler Thromb Vasc Biol 38:880-891 (2018). PubMed: 29496660
- Liu Q et al. Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells. Stem Cells Transl Med 1:266-78 (2012). ICC/IF ; Human . PubMed: 23197806