Application
Western blot
Sample
Mouse Cell lysate - other (pancreatic islet lysate, membrane fraction)
Loading amount
18 µg
Specification
pancreatic islet lysate, membrane fraction
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/750
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T + 0.5%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG Antibody, AP182P
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100
Detection
Detection method
ECLprime
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
Additional data
PubMed ID
22918877: View
Additional Notes
The specificity of the band was verified by using the Dopamine Receptor D3 peptide (ab128688).
lane 1: pancreatic islet lysate, membrane fraction
lane 2: pancreatic islet lysate, cytosolic fraction
lane 3: pancreatic islet lysate, nuclear fraction
lane 1: pancreatic islet lysate, membrane fraction
lane 2: pancreatic islet lysate, cytosolic fraction
lane 3: pancreatic islet lysate, nuclear fraction
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投稿 Jan 17 2013