Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: R°C
Antigen retrieval step
None - Buffer/Enzyme Used: citrate buffer pH6
Sample
Sheep Tissue sections (Sheep pituitary)
Specification
Sheep pituitary
Permeabilization
No
Fixative
Bouins
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking serum
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500
Additional data
Additional Notes
Double immunostaining of D1DR (Red) and alpha GSU (Green) in sheep pituitary.
When testing ab20066 with a newer lot, I could not reproduce the same results.
Abcam response
Thank you for your valuable feedback. Sheep was not a tested species for ab20066. The present results suggest all lots may not be suitable for staining sheep tissues. Abcam would welcome feedback from other customers using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 10 2014