Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab97082)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, WB, IHC-P, ELISA
製品の概要
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製品名
Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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アプリケーション
適用あり: ICC, WB, IHC-P, ELISAmore details -
吸着処理血清
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
免疫原
Other Immunogen Type corresponding to Rabbit IgG.
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標識
Alkaline Phosphatase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97082の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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WB |
1/2500 - 1/25000. (Chemiluminescent)
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IHC-P |
1/100 - 1/1000.
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ELISA |
1/2500 - 1/25000. (Primary ELISA)
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特記事項 |
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ICC
Use at an assay dependent dilution. |
WB
1/2500 - 1/25000. (Chemiluminescent) |
IHC-P
1/100 - 1/1000. |
ELISA
1/2500 - 1/25000. (Primary ELISA) |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab97082 は 3 報の論文で使用されています。
- Matsuoka T et al. Inhibition of NLRP3 inflammasome by MCC950 improves the metabolic outcome of islet transplantation by suppressing IL-1ß and islet cellular death. Sci Rep 10:17920 (2020). PubMed: 33087823
- Rigon L et al. Targeting Brain Disease in MPSII: Preclinical Evaluation of IDS-Loaded PLGA Nanoparticles. Int J Mol Sci 20:N/A (2019). PubMed: 31022913
- Ligaba-Osena A et al. Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana). Sci Rep 7:17104 (2017). WB . PubMed: 29213132