Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
Key features and details
- Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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アプリケーション
適用あり: Flow Cyt, IHC-P, ICC/IF, WBmore details -
吸着処理血清
Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
標識
DyLight® 488. Ex: 493nm, Em: 518nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/50 - 1/200.
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IHC-P |
1/50 - 1/500.
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ICC/IF |
1/50 - 1/500.
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|
WB |
1/1000 - 1/2000. Predicted molecular weight: 36 kDa.
5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. |
特記事項 |
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Flow Cyt
1/50 - 1/200. |
IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
WB
1/1000 - 1/2000. Predicted molecular weight: 36 kDa. 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended. |
画像
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All lanes : cocktail of mouse anti-hnRNP
, rabbit anti-actin, and goat anti-GAPDH at 1 µg/ml
All lanes : Hela
Secondary
All lanes : cocktail of Dylight® 680-conjugated donkey anti-mouse
(green), Dylight® 800-
conjugated donkey anti-rabbit (red), and Dylight® 488-conjugated
donkey anti-goat (blue) (ab96935) at 0.5 µg/ml
Predicted band size: 36 kDa -
Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab96935 は 7 報の論文で使用されています。
- Berg IC et al. Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D. Adv Healthc Mater 10:e2100223 (2021). PubMed: 33890430
- Gentile SD et al. Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids. Front Bioeng Biotechnol 8:792 (2020). PubMed: 32793571
- Kaylan KB et al. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling. Elife 7:N/A (2018). PubMed: 30589410
- Compeer EB et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun 9:1597 (2018). PubMed: 29686427
- Rodriguez AM & Downs KM Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula. Dev Biol N/A:N/A (2017). PubMed: 28882402
- Kawamura M et al. Tissue-engineered smooth muscle cell and endothelial progenitor cell bi-level cell sheets prevent progression of cardiac dysfunction, microvascular dysfunction, and interstitial fibrosis in a rodent model of type 1 diabetes-induced cardiomyopathy. Cardiovasc Diabetol 16:142 (2017). PubMed: 29096622
- Kaylan KB et al. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFß, and extracellular matrix. Sci Rep 6:23490 (2016). PubMed: 27025873