Anti-Dnmt1 抗体 (ab87654)
Key features and details
- Rabbit polyclonal to Dnmt1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Dnmt1 antibody
Dnmt1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dnmt1 -
由来種
Rabbit -
特異性
ab87656 is recommended for DNMT1 detection in WB. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form
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特記事項
Useful for analysis of the protein complex containing Dnmt1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Traces of Ammonium sulphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Useful for analysis of the protein complex containing Dnmt1. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/5000.
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WB |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa).
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特記事項 |
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ICC/IF
1/5000. |
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa). |
ターゲット情報
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機能
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. -
組織特異性
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. -
配列類似性
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger. -
ドメイン
The N-terminal part is required for homodimerization and acts as a regulatory domain. -
翻訳後修飾
Sumoylated; sumoylation increases activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1786 Human
- Entrez Gene: 13433 Mouse
- Omim: 126375 Human
- SwissProt: P26358 Human
- SwissProt: P13864 Mouse
- Unigene: 202672 Human
- Unigene: 128580 Mouse
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別名
- ADCADN antibody
- AIM antibody
- CXXC finger protein 9 antibody
see all
画像
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ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
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All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Tagged-NP 95 + BirA Input
Lane 2 : Tagged-NP 95 + BirA Beads
Lane 3 : BirA Input
Lane 4 : BirA Beads
Predicted band size: 183 kDa
Observed band size: 183 kDa
Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna. -
All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
Lane 4 : BirA (0.1M KCl fraction)
Predicted band size: 183 kDa
Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab87654 は 25 報の論文で使用されています。
- Uysal F et al. Embryo culture media differentially alter DNA methylating enzymes and global DNA methylation in embryos and oocytes. J Mol Histol 53:63-74 (2022). PubMed: 34741214
- Uysal F et al. DNMT enzymes differentially alter global DNA methylation in a stage-dependent manner during spermatogenesis. Andrologia 54:e14357 (2022). PubMed: 34997784
- Bayerl J et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28:1549-1565.e12 (2021). PubMed: 33915080
- Haggerty C et al. Dnmt1 has de novo activity targeted to transposable elements. Nat Struct Mol Biol 28:594-603 (2021). PubMed: 34140676
- Wang W et al. Mutation-induced DNMT1 cleavage drives neurodegenerative disease. Sci Adv 7:eabe8511 (2021). PubMed: 34516921