Anti-Dihydrofolate reductase (DHFR) 抗体 [EPR5284] (ab133546)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284]
Dihydrofolate reductase (DHFR) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. corresponding to Human Dihydrofolate reductase (DHFR) aa 1-100.
Database link: P00374 -
ポジティブ・コントロール
- HeLa, Jurkat, U937, 293T, C6, RAW 264.7 and PC-12 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 1.28 x 10 -10 M Learn more about KD -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5284 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133546の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
ターゲット情報
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機能
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. -
パスウェイ
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. -
配列類似性
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1719 Human
- Entrez Gene: 13361 Mouse
- Entrez Gene: 24312 Rat
- Omim: 126060 Human
- SwissProt: P00374 Human
- SwissProt: P00375 Mouse
- SwissProt: Q920D2 Rat
- Unigene: 592364 Human
see all -
別名
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
see all
画像
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All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution (purified)
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U937 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab133546 は 7 報の論文で使用されています。
- Lee SY et al. Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies. Biotechnol J 16:e2000351 (2021). PubMed: 33314785
- Tordonato C et al. miR-146 connects stem cell identity with metabolism and pharmacological resistance in breast cancer. J Cell Biol 220:N/A (2021). PubMed: 33819341
- Gong L et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). PubMed: 30975976
- Ashkavand Z et al. Metabolic Reprogramming by Folate Restriction Leads to a Less Aggressive Cancer Phenotype. Mol Cancer Res 15:189-200 (2017). WB ; Mouse . PubMed: 28108628
- Beane OS et al. Disparate Response to Methotrexate in Stem Versus Non-Stem Cells. Stem Cell Rev 12:340-51 (2016). PubMed: 26815725
- Thoms JA et al. Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr. Mol Cell Biol 36:1222-36 (2016). PubMed: 26830229
- Lee SW et al. Overexpression of thymidylate synthetase confers an independent prognostic indicator in nasopharyngeal carcinoma. Exp Mol Pathol 95:83-90 (2013). IHC ; Human . PubMed: 23726796