Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver, lung)
Specification
liver, lung
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Boehringer Blocking reagent as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: water
Secondary antibody
Name
Non-Abcam antibody was used: anti goat HRP polymer
Host species: Rat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
real application: ISH on FFPE tissue (of lung and liver)
fixation: formaldehyde
pretreatment: proteinase K
Blocking: blocking reagent from Boehringer, undiluted for 30 min on RT
ab 76907: works in dilution 1/1000-1/32000, diluted in water
secondary antibody: anti goat, host: N/A (it's a HRP conjugated polymer)
image: human/ISH
fixation: formaldehyde
pretreatment: proteinase K
Blocking: blocking reagent from Boehringer, undiluted for 30 min on RT
ab 76907: works in dilution 1/1000-1/32000, diluted in water
secondary antibody: anti goat, host: N/A (it's a HRP conjugated polymer)
image: human/ISH
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投稿 Aug 21 2012