Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Permeabilization
Yes - 0.2% Triton X100
Specification
HeLa cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: blocking buffer
Dilution
1/50
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti-rabbit AlexaFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
I have tried the non-purified version of this product in the past and the purified version surpassed all expectations. This antibody works great in immunofluorescence. Granted, I had to use it at a 1:50 dilution but the tubulin looked beautiful. Detyrosinated tubulin is increased in Chlamydia infected HeLa cells and you can see clear tubules around these cells. Highly recommended.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Fabienne Paumet
Verified customer
投稿 Apr 27 2018