Anti-Desmoplakin I+II 抗体 (ab71690)
Key features and details
- Rabbit polyclonal to Desmoplakin I+II
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Desmoplakin I+II antibody
Desmoplakin I+II 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Desmoplakin I+II -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Desmoplakin I+II aa 1-100.
(Peptide available asab71689) -
ポジティブ・コントロール
- This antibody gave a positive signal in Caco 2 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71690の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 238, 270 kDa (predicted molecular weight: 260 , 332 kDa).
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 238, 270 kDa (predicted molecular weight: 260 , 332 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. -
組織特異性
Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. -
関連疾患
Keratoderma, palmoplantar, striate 2
Cardiomyopathy, dilated, with woolly hair and keratoderma
Arrhythmogenic right ventricular dysplasia, familial, 8
Skin fragility-woolly hair syndrome
Epidermolysis bullosa, lethal acantholytic
Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis -
配列類似性
Belongs to the plakin or cytolinker family.
Contains 17 plectin repeats.
Contains 1 SH3 domain.
Contains 6 spectrin repeats. -
ドメイン
Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments. -
翻訳後修飾
Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377). -
細胞内局在
Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 1832 Human
- Omim: 125647 Human
- SwissProt: P15924 Human
- Unigene: 519873 Human
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別名
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- DCWHKTA antibody
- Desmoplakin (DPI DPII) antibody
see all
画像
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ab71690 staining Desmoplakin I+II in HaCat cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab71690 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Desmoplakin I+II antibody (ab71690) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 260 , 332 kDa
Observed band size: 238,270 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab71690 は 19 報の論文で使用されています。
- Castel G & David L Induction of human trophoblast stem cells. Nat Protoc 17:2760-2783 (2022). PubMed: 36241723
- Nath A et al. Palmitate-Induced IRE1-XBP1-ZEB Signaling Represses Desmoplakin Expression and Promotes Cancer Cell Migration. Mol Cancer Res 19:240-248 (2021). PubMed: 33106375
- Zhang K et al. Plakophilin-2 truncating variants impair cardiac contractility by disrupting sarcomere stability and organization. Sci Adv 7:eabh3995 (2021). PubMed: 34652945
- Nedder M et al. Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32635405
- Shoaito H et al. Peroxisome proliferator-activated receptor gamma-ligand-binding domain mutations associated with familial partial lipodystrophy type 3 disrupt human trophoblast fusion and fibroblast migration. J Cell Mol Med 24:7660-7669 (2020). PubMed: 32519441