Anti-Desmoplakin I+II 抗体 (ab118804)
Key features and details
- Rabbit polyclonal to Desmoplakin I+II
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Desmoplakin I+II antibody
Desmoplakin I+II 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Desmoplakin I+II -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IP: HeLa whole cell lysate; IHC: human prostate carcinoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab118804 was affinity purified using an epitope specific to Desmoplakin I+II immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. -
組織特異性
Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. -
関連疾患
Keratoderma, palmoplantar, striate 2
Cardiomyopathy, dilated, with woolly hair and keratoderma
Arrhythmogenic right ventricular dysplasia, familial, 8
Skin fragility-woolly hair syndrome
Epidermolysis bullosa, lethal acantholytic
Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis -
配列類似性
Belongs to the plakin or cytolinker family.
Contains 17 plectin repeats.
Contains 1 SH3 domain.
Contains 6 spectrin repeats. -
ドメイン
Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments. -
翻訳後修飾
Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377). -
細胞内局在
Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 1832 Human
- Omim: 125647 Human
- SwissProt: P15924 Human
- Unigene: 519873 Human
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別名
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- DCWHKTA antibody
- Desmoplakin (DPI DPII) antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Demoplakin I+II with ab118804 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Desmoplakin I+II was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab118804 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using a rabbit anti-Desmoplakin antibody which recognizes an upstream epitope at 1 µg/mL.
Lane 1: ab118804 IP in HeLa whole cell lysate.
Lane 2: rabbit anti-Desmoplakin antibody recognizing upstream epitope IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab118804 は 1 報の論文で使用されています。
- Li P et al. Nuclear localization of Desmoplakin and its involvement in telomere maintenance. Int J Biol Sci 15:2350-2362 (2019). PubMed: 31595153