Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Loading amount
40 µg
Specification
MDA-MB-231 breast cancer cell line
Gel Running Conditions
Reduced Denaturing (gel 6 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 330, 310 kDa Non-specific: 80 kDa
Positive control
MCF7, A431 cells
Additional data
Additional Notes
Arrows indicate two specific anti-Desmoplakin bands recognized by the ab16434 antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 08 2008