Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (Huh7 liver)
Specification
Huh7 liver
Permeabilization
Yes - tritonx100
Fixative
Paraformaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: 1%BSA in PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Alexaflour 594
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
These cells were infected with dengue 2 NGC strain for 24 hours then fixed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 15 2014