Anti-DeltaD 抗体 [Dld 5C7/2, zdd2] (ab73331)
Key features and details
- Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD
- Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IHC - Wholemount, Flow Cyt
- Reacts with: Zebrafish
- Isotype: IgG1
製品の概要
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製品名
Anti-DeltaD antibody [Dld 5C7/2, zdd2] -
製品の詳細
Mouse monoclonal [Dld 5C7/2, zdd2] to DeltaD -
由来種
Mouse -
特異性
This antibody recognises the extracellular domain of zebrafish DeltaD. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA. -
アプリケーション
適用あり: WB, ELISA, ICC/IF, IHC-Fr, IHC - Wholemount, Flow Cytmore details -
種交差性
交差種: Zebrafish -
免疫原
Extracellular DeltaD peptide (Zebrafish).
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エピトープ
Zebrafish DeltaD protein extracellular domain. -
ポジティブ・コントロール
- HEK293 cells transfected with deltaD or zebrafish embryo.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Dld 5C7/2, zdd2 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73331の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC - Wholemount | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC - Wholemount
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
DeltaD is one of the 4 members of the zebrafish Delta family of Notch ligands, important in the control of cell differentiation in many tissues. DeltaD acts as a ligand for Notch receptors and is involved in primary neurogenesis and somitogenesis. It can activate Notch receptors, thereby playing a key role in lateral inhibition, a process that prevents the immediate neighbors of each nascent neural cell from simultaneously embarking on neural differentiation. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 30138 Zebrafish
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別名
- Aei antibody
- After eight antibody
- Dld antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab73331 は 11 報の論文で使用されています。
- Zhao X et al. Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division. Sci Adv 7:N/A (2021). WB, ICC ; Zebrafish . PubMed: 34117063
- Sehgal P et al. LncRNA VEAL2 regulates PRKCB2 to modulate endothelial permeability in diabetic retinopathy. EMBO J 40:e107134 (2021). PubMed: 34180064
- Sharma P et al. Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis. Sci Rep 9:9958 (2019). PubMed: 31292468
- Gallagher TL et al. Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Dev Biol 429:225-239 (2017). PubMed: 28648842
- Tong K et al. Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo. Methods Mol Biol N/A:N/A (2017). PubMed: 28434166