Anti-DeltaC 抗体 [zdc2] (ab73336)
Key features and details
- Mouse monoclonal [zdc2] to DeltaC
- Suitable for: ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, WB
- Reacts with: Zebrafish
- Isotype: IgG2a
製品の概要
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製品名
Anti-DeltaC antibody [zdc2] -
製品の詳細
Mouse monoclonal [zdc2] to DeltaC -
由来種
Mouse -
特異性
Delta proteins activate Notch through a binding reaction that depends on their extracellular domains. During somitogenesis, Notch signalling via DeltaC keeps the oscillations of neighbouring cells in synchrony. DeltaC is also expressed in subsets of cells in the nervous system and elsewhere. -
アプリケーション
適用あり: ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, WBmore details -
種交差性
交差種: Zebrafish -
免疫原
Extracellular region of DeltaC protein (Zebrafish).
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エピトープ
DeltaC extracellular domain. -
ポジティブ・コントロール
- HEK293 cells transfected with DeltaC or zebrafish embryo cryosections (formulin fixed).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
zdc2 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73336の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
|
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IHC - Wholemount | (1) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC - Wholemount
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
DeltaC acts as a ligand for Notch receptors and is involved in somitogenesis. It can activate Notch receptors. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized. -
細胞内局在
Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 30120 Zebrafish
- SwissProt: Q9IAT6 Zebrafish
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別名
- Bea antibody
- Beamter antibody
- DelC antibody
- Dlc antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab73336 は 5 報の論文で使用されています。
- Sehgal P et al. LncRNA VEAL2 regulates PRKCB2 to modulate endothelial permeability in diabetic retinopathy. EMBO J 40:e107134 (2021). PubMed: 34180064
- Tietz KT et al. Pumilio response and AU-rich elements drive rapid decay of Pnrc2-regulated cyclic gene transcripts. Dev Biol 462:129-140 (2020). PubMed: 32246943
- Gallagher TL et al. Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Dev Biol 429:225-239 (2017). PubMed: 28648842
- Quillien A et al. Distinct Notch signaling outputs pattern the developing arterial system. Development 141:1544-52 (2014). Zebrafish . PubMed: 24598161
- Giudicelli F et al. Setting the tempo in development: an investigation of the zebrafish somite clock mechanism. PLoS Biol 5:e150 (2007). PubMed: 17535112