Application
Flow Cytometry
Sample
Mouse Cell (RAW and J774 murine macrophages)
Specification
RAW and J774 murine macrophages
Preparation
Cell harvesting/tissue preparation method: routine cell culture --> scraped, washed
Sample buffer: PBS + FBS (3%)
Sample buffer: PBS + FBS (3%)
Fixation
None
Permeabilization
No
Gating Strategy
Size gating of murine RAW cells
Other product details
Dilution
1/10
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: PBS + 3% FBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Critical issues are 3 fold. First, you have to block with Fcg blocking antibody -- murine cells will suck up your isotype otherwise. Second, use an isotype. Finally, the antibody will not last indefinately -- the last time I used it (yesterday) it had lst activity -- but is > 1 year old
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 23 2008