Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 23°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit TxR
Host species: Donkey
Clonality: Polyclonal
Conjugation: PE/Texas Red ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: PE/Texas Red ®
Dilution
1/150
Additional data
Additional Notes
No/few signal in the tested conditions.
DYRK1A in red, microtubules in green, DNA in blue.
DYRK1A in red, microtubules in green, DNA in blue.
Abcam response
Texas Red has been reported to produce weak signal if used in conjunction with other fluorescence dyes, it might be useful to try a different secondary antibody in order to increase the fluorescence signal. Thank you for your valuable feedback, we hope this will be useful for other customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 20 2009