Anti-DYNLT3 抗体 (ab121209)
Key features and details
- Rabbit polyclonal to DYNLT3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DYNLT3 antibody -
製品の詳細
Rabbit polyclonal to DYNLT3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
antigen sequence: DEVGFNAEEA HNIVKECVDG VLGGEDYNHN NINQWTASIV EQSLTHLVKL GKAYKYIVTC AVVQKSAYGF HTASSCFWDT TSDGTCTVRW E, corresponding to amino acids 9-99 of Human DYNLT3.
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ポジティブ・コントロール
- WB: Wild-type HeLa, SK-MEL-20, MCF-7, and NBT-II whole cell lysates; IHC-P: Human cerebral cortex tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121209の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Probably binds BUB3 as part of transport cargo. Required for the efficient progression through mitosis. -
配列類似性
Belongs to the dynein light chain Tctex-type family. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton. Chromosome > centromere > kinetochore. Colocalizes with BUB3 at kinetochores specifically during prometaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 6990 Human
- Entrez Gene: 67117 Mouse
- Entrez Gene: 363448 Rat
- Omim: 300302 Human
- SwissProt: P51808 Human
- SwissProt: P56387 Mouse
- Unigene: 446392 Human
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別名
- 2310075M16Rik antibody
- AU042796 antibody
- DYLT3_HUMAN antibody
see all
画像
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All lanes : Anti-DYNLT3 antibody (ab121209) at 0.04 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DYNLT3 knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 13 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-DYNLT3 antibody (ab121209) staining at 0.04 µg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab121209 was shown to bind specifically to DYNLT3. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in DYNLT3 CRISPR-Cas9 edited cell lysates. The band observed in the CRISPR-Cas9 edited lysate lane above 13 kDa is likely to represent DYNLT3 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DYNLT3 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-DYNLT3 antibody (ab121209)
Lane 1 : SK-MEL-30 (human melanoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysateLoading control: Anti-PFN1.
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ab121209 at 1/750 dilution staining DYNLT3 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
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All lanes : Anti-DYNLT3 antibody (ab121209)
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat urinary bladder tumor cell line) whole cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab121209 は 5 報の論文で使用されています。
- Zhang J et al. DYNLT3 overexpression induces apoptosis and inhibits cell growth and migration via inhibition of the Wnt pathway and EMT in cervical cancer. Front Oncol 12:889238 (2022). PubMed: 35965516
- Lai KY et al. A Ran-binding protein facilitates nuclear import of human papillomavirus type 16. PLoS Pathog 17:e1009580 (2021). PubMed: 33974675
- Gu X et al. Age-associated genes in human mammary gland drive human breast cancer progression. Breast Cancer Res 22:64 (2020). PubMed: 32539762
- Liu Y et al. Nigrostriatal dynein changes in A53T alpha-synuclein transgenic mice. F1000Res 3:68 (2014). PubMed: 25210616
- Chu Y et al. Alterations in axonal transport motor proteins in sporadic and experimental Parkinson's disease. Brain 135:2058-73 (2012). ICC ; Rat . PubMed: 22719003