Our lab uses DRAQ5 from Abcam for the In Cell Western Assay. DRAQ5 is used to provide accurate normalization over a broad range of cell densities. Specifically, it can be used for stoichiometric staining of DNA in live or fixed cells. DRAQ5 is used in the 700nm channel to normalize well-to well variation in cell number.
Picture shows In Cell Wester Assay using DRAQ5 in PC3 and LnCAP prostate cell lines.
Picture shows In Cell Wester Assay using DRAQ5 in PC3 and LnCAP prostate cell lines.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 25 2014