Anti-DRAK2 抗体 (ab8419)
Key features and details
- Rabbit polyclonal to DRAK2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-DRAK2 antibody
DRAK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DRAK2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
CSKRFRFDDSLPNPHE
, corresponding to amino acids 351/365 of Human DRAK2. (Peptide available as ab8454.) -
ポジティブ・コントロール
- Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.
-
特記事項
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
精製度
Ion Exchange Chromatography -
特記事項(精製)
DRAK2 Antibody is Ion exchange chromatography purified. -
一次抗体 備考
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8419の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
特記事項 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Acts as a positive regulator of apoptosis. -
組織特異性
Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 9262 Human
- Omim: 604727 Human
- SwissProt: O94768 Human
- Unigene: 88297 Human
-
別名
- DAP kinase related apoptosis inducing protein antibody
- DAP kinase related apoptosis inducing protein kinase 2 antibody
- DAP kinase-related apoptosis-inducing protein kinase 2 antibody
see all
画像
-
Lane 1 : Anti-DRAK2 antibody (ab8419) at 1 µg/ml (DRAK2 antibody)
Lane 2 : Anti-DRAK2 antibody (ab8419) at 2 µg/ml (DRAK2 antibody)
All lanes : Raji cell lysate
Predicted band size: 43 kDa -
All lanes : Anti-DRAK2 antibody (ab8419) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate with absence of blocking peptide
Lane 2 : Raji whole cell lysate with absence of blocking peptide
Lane 3 : Jurkat whole cell lysate with presence of blocking peptide
Lane 4 : Raji whole cell lysate with presence of blocking peptide
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
We are unsure about the nature of the 70 kDa band. However, DRAK2 is autophosphorylated and it is possible that this band corresponds to the phosphorylated form. The fact that the detection of this band is blocked by DRAK2 peptide indicates that it probably is closely related to the DRAK2 protein. -
ab8419 at 10µg/ml staining DRAK2 in Jurkat cells by ICC/IF
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab8419 は論文での使用が確認できていません。