Application
Western blot
Sample
Human Recombinant protein (from human liver cDNA library, baculovirus infecte)
Loading amount
0.32 µg
Specification
from human liver cDNA library, baculovirus infecte
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Blocking step
0.05 M Tris buffer containing 0.15 M NaCl, 5 mM NaN3 and 2% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05 M Tris, 0.15 M NaCl, 0.1% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 117 kDa
Positive control
lane 4: recombinant DPP8
Negative control
lane 1: molecular weight standards
lane 2: human DPPII
lane 3: human DPPIV
lane 5: bovine DPP9
lane 6: molecular weight standards
lane 2: human DPPII
lane 3: human DPPIV
lane 5: bovine DPP9
lane 6: molecular weight standards
Additional data
PubMed ID
19188489: View
Additional Notes
nitrocellulose membrane
primary antibody was incubated for 1 hr at room temperature and overnight at 4°C
primary antibody was incubated for 1 hr at room temperature and overnight at 4°C
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Veronique Dubois
Verified customer
投稿 Jun 11 2009